天然羟基磷灰石圆盘状试样的制备及细胞在其表面的培养
王健丹，张志伟，吕晓迎
（东南大学生物电子学国家重点实验室，南京，210096）
摘要：本研究的目的是研究天然羟基磷灰石的生物相容性。首先采用高温煅烧法从猪骨中制备天然羟基磷灰石，然后压制成圆盘状试样。接着，在体外培养小鼠骨髓间充质干细胞，并检测其细胞活力代谢标准曲线，最后对小鼠间充质干细胞在NHA圆盘状试样表面的生长状况进行研究。结果显示650°C煅烧能够从猪骨中获得NHA，骨髓间充质干细胞的活力代谢标准曲线的研究结果表明适宜的细胞种板浓度为6000细胞/孔；细胞在NHA圆盘状试样表面生长状态良好，这表面NHA具有良好的生物相容性。
关键词：生物材料；天然羟基磷灰石；间充质干细胞；细胞活力代谢标准曲线；生物相容性
中图分类号：R318.08

 Preparation of Disk-like Natural Hydroxyapatite Samples and Cell Culture on Their Surfaces
WANG Jiandan, ZHANG Zhiwei, Lü Xiaoying
(State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096)
Foundations: Doctoral Program of Higher Education of China (No.20100092110027); 973 Project (No. 2009CB930000); the National Natural Science Foundation of China (No. 31170910, 31271012, 30770583); the Graduate Research and Innovation Program of Jiangsu Province (No. CX10B-082Z) Brief author introduction:王健丹(1987-),女，硕士，主要研究方向：生物材料，生物相容性 Correspondance author: 吕晓迎(1956-),女，教授，主要研究方向：生物材料，生物相容性，生物电子学.

Abstract: The purpose of this study is to prepare natural hydroxyapatite (NHA) for biocompatibility study. The NHA was prepared from pig bones by means of a calcining procedure, and then were made into disk-like samples. Subsequently mouse mesenchymal stem cells (MSCs) were cultured in vitro and the cell viability metabolism standard curve was obtained. Finally the mouse MSCs were also tried to seed on the surface of disk-like NHA samples. The result showed NHA could be derived from pig bones by a 650°C calcining procedure. MSCs viability metabolism standard curve was obtained and proper cell density of 6000 cells per wells was selected. The cells grew well on the surface of disk-like NHA samples. These results demonstrated that NHA have good biocompatibility.
Key words: Biomaterial; Natural hydroxyapatite (NHA); Mesenchymal stem cells (MSCs); Cell  viability metabolism standard curve; Biocompatibility