碳纳米管对血液中重要蛋白的影响
封如，焦延鹏，周长忍
基金项目：教育部博士点新教师基金资助项目(20094401120006)；广东省科优秀博士论文项目(sybzzxm201034)
作者简介：封如（1986-），女，在读研究生，主要研究方向：生物材料
通信联系人：焦延鹏（1975-），男，研究员，主要研究方向：生物材料.

（暨南大学材料科学与工程系）
摘要：碳米管材料近年来备受关注，广泛应用于药物传导、核酸传导、光学治疗和光声分子成像等领域。碳米管主要是通过血液循环系统进入人体，会影响多种血液组分的生物功能。血清蛋白、γ-球蛋白和纤维蛋白原是人体血液中的三种主要蛋白，纳米管材料进入血液后，会不可避免地与这三种蛋白相互作用，影响这些蛋白的结构与功能。为了实现纳米管的临床应用，有必要研究它们与这些血液蛋白之间的相互作用机理。本文研究了单壁碳纳米管在不同浓度（0.001-0.5mg/mL）下对血清蛋白、γ-球蛋白和纤维蛋白原连续作用2 小时后，使用了紫外分光光谱、荧光光谱和圆二色谱分析了蛋白结构的变化。实验结果表明，碳纳米管对蛋白的影响与投入浓度是正相关的；在某一低浓度范围（≤0.05mg/mL）内，纳米管不会对血清蛋白、γ-球蛋白、纤维蛋白原的结构和功能产生影响。该实验结果为碳米管材料在某一浓度范围内的临床应用提供了有效信息。
关键词：碳纳米管；血液蛋白；血液相容性；
中图分类号：R944.9
Effect of carbon nanotube on the structure of major proteins in blood
Feng Ru, Jiao Yanpeng, Zhou Changren
(Department of Materials Science and Engineering, Jinan University, Guang Zhou 510632)
Abstract: Nanotube materials are recently receiving considerable attention in drug delivery,nucleic acid delivery, biomaterials therapy and photo acoustic molecular bio-imaging. For in vivo application, nanotubes will enter blood circulation and interact with various blood components and to affect their bio-functions. Serum albumin, γ-Globulin and Fibrinogen are three major proteins in human blood. The interaction of nanotubes with these proteins are unavoidable, affecting the
ructure and fuction of the proteins and even the whole organism. So this effect should be clearly elucidated at cellular and molecular levels. In this study, the effect of Single-Wall Carbon Nanotubes (CNT) on Bovine Serum Albumin (BSA), γ -Globulin and Fibrinogen were investigated. The proteins were exposed to nanotubes for 2 hours at different concentrations (0.001-0.5mg/mL). The results showed that the effect on proteins was positively correlated with the exposed nanotubes' concentration. When the concentration was less than 0.05mg/mL, nanotubes led to no significant negative inpact on the structure and fuction of the proteins. The data provide useful information for further clinical applications of nanotube materials.
Key words: Carbon nanotubes; Blood proteins; Hemocompatibility