整合素α5在食管鳞癌组织中的表达及其临床意义
郭津丞1，陈博2，吴健谊1，吴智勇1,3，许秀娥2，许丽艳2，李恩民1，冉利强1，谢剑君1
基金项目：中国高等教育博士点科研基金项目新教师联合类（20104402120005） 作者简介：郭津丞（1989-），男，硕士研究生，肿瘤分子生物学 通信联系人：谢剑君（1979-），男，副教授，肿瘤分子生物学.

（1. 汕头大学医学院生物化学与分子生物学教研室，汕头 515041； 2. 汕头大学医学院肿瘤病理研究室，汕头 515041； 3. 汕头市中心医院肿瘤科 汕头 515031）
摘要：目的 研究整合素α5（Integrin α5）在食管鳞癌组织中的表达及其与临床病理参数10 的关系。方法 应用组织芯片和免疫组织化学染色等方法，检测了整合素α5在147例食管鳞癌组织中的表达情况并分析了其表达与临床病理参数以及患者预后的关系。结果 1）免疫组化染色提示，在147例食管鳞癌组织中，整合素α5在癌细胞呈强染色的为34例（>5分，23.1%），弱或中等强度染色有113 例（76.9%）；2）整合素α5的表达与食管鳞癌组织的分化程度显著相关；3）整合素α5的表达水平与食管癌患者的生存率呈显著负相关15 （P=0.005），整合素α5的表达可以作为判断食管癌预后的独立预警分子（P=0.003）。 结论 整合素α5可能在食管癌发生发展过程中发挥重要作用。
关键词：食管鳞癌；整合素α5；患者预后
中图分类号：R446.8

Expression of Integrin α5 in esophageal squamous cell carcinoma
GUO Jincheng1, CHEN Bo2, WU Jianyi1, WU Zhiyong1,3, XU Xiue2, XU Liyan2, LI Enmin1, RAN Liqiang1, XIE Jianjun1
(1. Department of Biochemistry and Molecular Biology, Medical College of Shantou University, 25 Shantou 515041; 2. Institute of Oncologic Pathology, Medical College of Shantou University, Shantou 515041; 3. Department of Surgical Oncology, Shantou Central Hospital, Shantou 515031)
Abstract: Purpose: This study is aimed to explore the expression and the clinical significance of Integrin α5 in esophageal squamous cell carcinoma (ESCC). Methods: Integrin α5 expression was 30 evaluated using tissue microarray and immunohistochemical staining in 147 samples of human ESCC. Impact of Integrin α5 expression on the survival of ESCC patients was also determined. Results: Integrin α5 was high-expression in 23.1% (34/147) of the ESCC samples and was weak or moderate-expression in the other 113 cases. Integrin α5 expression was associated with differentiation of ESCC. Moreover, high tumorous Integrin α5 expression was significantly 35 correlated with shorter overall survival and Multivariate Cox regression analysis revealed that Integrin α5 expression was an independent prognostic factor for human ESCC patients. Conclusions: Integrin α5 is an ESCC candidate clinical prognostic marker and a potential treatment target.
Key words: esophageal squamous cell carcinoma; Integrin α5; prognostic factor