摘要：种植体的表面微结构时决定细胞以及细菌行为的重要因素，但目前哪种微结构对上述行为有着积极影响尚不清楚。在本文中，我们分别在羟基磷灰石以及聚二甲基硅氧烷片上制备了不同形状以及不同尺寸的微结构，并将C2C12 细胞以及S.a 、P.g 两种细菌分别在上述两种材料上培养，以分析细胞以及细菌在不同微结构上的表现。结果表明，C2C12 细胞在HA 上或者亚微米结构上生长较好，相反的，细菌更倾向于在微米级的结构表面生长。
Investigation of Adhesion and Proliferation Behavior of Myoblast and Bacteria on Micro-pattern Chip
Bao Chongyun, Xiao Yu
(State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University,Chengdu, 610041)Foundations: Natural Science Foundation of China (No. 81371181, No. 81171005)， Ministry of Education,Fund for the Doctoral (No. 20100181110058)
Brief author introduction:Bao Chongyun(1967-),Male, Professor, The main research field is in-vivo tissue engineering for bone regeneration.
Abstract: Surface microstructure of implant materials is an essential factor to determine the adhesion and proliferation behaviors of cells and bacteria. However, it is still unclear which kind of morphological characteristics plays dominant roles. For this purpose, hydroxyapatite (HA) and polydimethylsiloxane (PDMS) chips were prepared firstly with different types of microstructures and the C2C12 cells were seeded on these chips to investigate which microstructures could affect the
behavior of cells. Next, Staphylococcus aureus(S.a) and Porphyromonas gingivalis(P.g) were cultured on the PDMS matrix, respectively, to analyze the performance of bacteria on varied surface microstructures. The results illustrated much more C2C12 cells seeded on the HA chips than the PDMS 15 replicas, indicating HA are more suitable for the cell’s culture. Moreover, further observation and comparison represented both HA and PDMS samples illustrated a similar trend that the cells mainly adhered and proliferated on the submicron structure region rather than microstructure region. By contrast, the bacteria were significantly preferable to the microstructure regions. Overall, on account of different performance of cells and bacteria, we drew the conclusion that the surface roughness (RA) of implant materials should be manufactured in submicron-scale rather than micro-scale for improving the biocompatibility of biomaterials.
Key words: Basic Science of Stomatology; Microstructure; Hydroxyapatite; Cells Behavior;Roughness
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