当前位置:首页 > 智慧健康列表 > 研发与技术创新 > 详细信息
远曲小管闰细胞和入球微动脉的直接接触:小鼠肾脏数字化三维重建
来源: | 作者: | 发布时间:2014-4-15 17:28:24

远曲小管闰细胞和入球微动脉的直接接触:小鼠肾脏数字化三维重建
任昊,翟效月
(中国医科大学,组织胚胎学教研室)
摘要:远端小管和入球小动脉之间的直接接触已经在大鼠和家兔报道过。除了致密斑外,该反馈机制的存在对全身酸碱和电解质状态的控制也有显著影响。在本研究中,我们应用计算机辅助三维肾追踪结合免疫组化方法研究在小鼠肾脏中是否存在类似的接触现象。在共168个追踪的肾单位中,大多数肾单位(短髓袢的约90%、长髓袢肾单位的有75 %)有这种入球小动脉和远端小管之间的直接接触。这些接触部位总是发生在远曲小管的最后10 %节段。值得注意的是,在接触部位的肾小管管壁上总能找到闰细胞,而且是nonA-nonB 类型的。闰细胞在远曲小管的这种密切联系表明,这些接触部位可能存在和致密斑处类似的调控机制,可能参与在后致密斑肾小管节段上的pH 值和电解液的浓度调控。
关键词:关键词1 组织胚胎学;关键词2 数字化三维重建;关键词3 小鼠肾脏
中图分类号:R329.1

Direct Physical Contact between Intercalated Cells in Distal Convoluted Tubule and the Afferent Arteriole in Mice#
REN Hao, Xiaoyue Zhai
(china medical university, histology 5 and embryology)

Foundations:Specialized Research Fund for the Doctoral Program of Higher Education (No. 20102104120020)
Brief author introduction:associate prof.

Abstract: IDirect physical contact between the distal tubule and the afferent arteriole has been reported in rats and rabbits. The existence of a feedback mechanism termed cross-talk similar to that at macular densa has been proposed, which has significant impact on the control of systemic acid-base and electrolytes status. In the present study, we applied computer assisted 3D nephron tracing combined  with immunohistochemistry to investigate such contacts in mice. With tracing, it became possible to determine whether the tubule and the contacting arteriole were associated to the same parent nephron
or not. We investigated a total of 168 nephrons from 3 mouse kidneys. The majority of the nephrons (approximately 90% of the short-loop and 75% of the long-loop nephrons) had direct physical contact between their own afferent arteriole and distal tubule. These contacts always occurred at the last 10% of the distal convoluted tubule. Type nonA-nonB intercalated cells were always found in the tubular wall at the contact site. In conclusion, the present work demonstrated the existence of direct
tubule-arteriole contact in mice. The close association of intercalated cells in the distal convoluted tubule suggests that these contacts may be the morphological basis for a cross-talk mechanism, which is potentially involved in the post-macula densa humeral pH and electrolyte regulation in the kidney.
Key words: histology and embryology; 3D digital reconstruction; mouse kidney