硫化氢对宫内香烟暴露致新生乳鼠延髓损伤的抗氧化和抗炎症保护作用
胡雅洁，聂黎虹，晏翔，李明强，陈丽，周华，郑煜
基金项目：教育部博士点基金（批准号20100181110048）；国家自然科学基金（批准号31271233）
作者简介：胡雅洁（1988-），女，研究生，主要研究方向：呼吸神经生物学
通信联系人：郑煜（1955-），男，教授，博士生导师，主要研究方向：呼吸神经生物学.

 （四川大学华西基础医学与法医学院生理学教研室，四川 成都 610041）
摘要：硫化氢（Hydrogen sulfide，H2S）作为一种神经保护因子可通过其抗氧化、抗炎症和抗凋亡作用在神经退行性疾病中发挥保护作用。我们前期研究结果表明，H2S 可以通过其抗凋亡作用对抗宫内香烟暴露致新生乳鼠延髓呼吸中枢的损伤。本实验旨在研究H2S 对宫内香烟暴露所致新生乳鼠延髓呼吸中枢损伤的保护作用，并探讨H2S 的保护作用机制是否与H2S 的抗氧化及抗炎症作用有关。将SD 孕鼠随机分为4 组（n=8）：正常对照组(Con)、香烟暴露组(CS)、香烟暴露组+硫氢化钠组(CS+NaHS)以及硫氢化钠组(NaHS)。采用HE 染色观察各组新生乳鼠的延髓组织神经元的病理改变情况；采用尼氏染色观察各组新生乳鼠的延髓组织呼吸相关核团神经元尼氏体的改变情况；采用生物化学方法，检测MDA 含量和SOD活性，评估各组对新生乳鼠延髓组织的氧化应激水平的影响；采用RT-PCR 法，检测延髓组组织中TNF-α、IL-1β、 IL-6mRNA 表达水平，观察各组对新生乳鼠延髓组织炎症水平的影响。结果表明：H2S 对宫内香烟暴露新生乳鼠延髓呼吸中枢的损伤具有保护作用，且这种保护作用可能是通过抗氧化和抗炎症途径来发挥作用。
关键词：硫化氢；宫内香烟暴露；延髓；氧化应激；炎症；新生乳鼠
中图分类号：R338.2, Q473

The anti-oxidant and anti-inflammation actions of hydrogen sulfide against injuries of medullary neurons of neonatal rats subjected to in utero cigarette smoke exposure
HU Yajie, NIE Lihong, YAN Xiang, LI Mingqiang, CHEN Li, ZHOU Hua, ZHENG Yu
(Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan
University, Chengdu, Sichuan, 610041)
Abstract: Hydrogen sulfide (H2S) serves as a neuroprotectant via anti-oxidant, anti-inflammation and anti-apotosis in neurodegenerative diseases. Our previous work has demonstrated that H2S, via the anti-apoptotic action, protected rat medullary respiratory centers from injuries that was sustained from in utero cigarette smoke exposure. The present work was carried out to further investigate whether the protective action of H2S is associated with its anti-oxidant and anti-inflammation against injuries of medullary neurons induced by in utero cigarette smoke exposure in neonatal rats. Pregnant SD rats were randomly divided into 4 groups, including control (Con), cigarette smoke exposure (CS), CS+NaHS (donor of H2S) and NaHS groups. HE staining was applied to observe the pathological changes of medullary neurons; Nissl’s staining was used to examine the damage on Nissl bodies of the neurons; biochemical methods were utilized to assess the degree of oxidative stress in medulla oblongata tissue by measuring MDA content and SOD activity; RT-PCR technique was used to measure the levels of TNF-α, IL-1β and  IL-6 mRNA. The results revealed that some medullary neurons of neonatal rats suffered from injuries by in utero cigarette smoke exposure and NaHS ameliorated the injuries of the neurons. Moreover, NaHS decreased MDA content and improved SOD activity of the medulla oblongata of CS neonatal rats. In addition, NaHS also reduced TNF-α, IL-1β and IL-6 mRNA expression of the medulla oblongata of CS neonatal rats. We concluded therefore that anti-oxidant and
anti-inflammation actions of H2S are indeed involved in protection of medullary neurons of neonatal rats against injuries induced by in utero cigarette smoke exposure.
Key words: Hydrogen sulfide; Cigarette smoke exposure; Medulla oblongata; Oxidative stress;Inflammation; Neonatal rat