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人工血管材料表面双重功能化修饰促进血管原位内皮化
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人工血管材料表面双重功能化修饰促进血管原位内皮化
吉清,崔云,张素爱,王佳宁,王志红,孔德领,赵强
基金项目:高等学校博士学科点专项科研基金(新教师类)(20100031120021);国家自然科学基金(青年基金)(81000680) 作者简介:吉清,1988,女,硕士研究生,小口径人工血管的表面修饰 通信联系人:赵强,1978,男,副教授,生物活性材料.

(南开大学生物活性材料教育部重点实验室,天津,300071)
摘要:在小口径人工血管的研究中,血栓形成和内膜增生是导致血管狭窄的主要原因,因此防止血栓形成和促进材料表面快速内皮化是人工血管研究的一个重要方向。本文利用β-环糊精(β-CD)与金刚烷(AD)之间的主客体识别超分子组装,对聚己内酯(PCL)材料表面进行了双重功能化修饰。首先,制备了表面接枝β-CD的PCL膜,进而通过β-CD与AD之间主客体识别作用,将两种不同功能分子修饰到PCL表面。其中一种是具有抗蛋白和血小板粘附的聚乙二醇(PEG),另一种是能够特异性粘附内皮祖细胞(EPCs)的多肽TPSLEQRTVYAK (TPS)。进一步,系统评价了功能化表面的亲疏水性和生物学性能,如纤维蛋白原粘附、血小板粘附和细胞的特异性捕捉等。实验结果表明,PEG功能化修饰能显著抑制血浆蛋白和血小板的粘附,从而降低了材料表面血栓形成的几率。同时,TPS多肽的修饰则赋予材料表面特异性捕捉EPC的功能。本文所构建的双重功能化修饰的材料表面作为人工血管植入后能够促进内皮化进程,是一种具有良好应用前景的新型血管材料。
关键词:功能高分子材料;表面功能化;主客体超分子组装;内皮化;抗生物污染性能
中图分类号:063-0

Surface functionalization of vascular grafts with the aim to promote in-situ endothelialization
Ji Qing, Cui Yun, Zhang Suai, Wang Jianing, Wang Zhihong, Kong Deling, Zhao Qiang
(Key Laboratory of Bioactive Materials,College of Life Science, Nankai University, 25 Tianjin,300071)
Abstract: In the case of small-diameter vascular grafts, the thrombosis and intimal hyperplasia is the main reason for occlusion after implantation. Hence, to develop materials with surface funtions to resist the thrombus formation and promote endothelialization is an important aspect in the research of vascular grafts.In this study, we developed a method for the dual functionalization of 30 poly(ε-caprolactone) (PCL) surface by the means of supramolecular assembly based on the host-guest inclusion complexation between β-cyclodextrin (β-CD) and admantane (AD). Firstyly, β-CD was grafted onto the PCL surface. Then two types of functional molecules were immobilized on the PCL surface through host-guest inclusion complexation between β-cyclodextrin (β-CD) grafted on the PCL surface and admantane (AD) motif at the chain end of 35 functional molecules. One functional molecule is polyethylene glycol (PEG), which is resistance to plasma protein and platelet adsorption, and the other is TPSLEQRTVYAK (TPS) peptide, which can specifically bind endothelial progenitor cells (EPCs).Finally, the hydrophilic/hydrophobic property of the functionalized surface as well as the fibrinogen adsorption, platelet adhesion, and specific capture of cells were systematically evaluated on the 40 functionalized surface. The results show that the presence of PEG has evidently inhibited adsorption of plasma protein and platelet adhesion, thus reducing the possibility of thrombus formation. At the same time, TPS-functionalized surface demonstrated selective adhesion towards EPC instead of other types of cells.All these results indicate that the dually functionalized surface developed in this study is a suitable candidate for vascular graft to induce or promote in-situ 45