表皮葡萄球菌色氨酰-tRNA合成酶抑制剂W-23的抗菌活性研究
吴旸，毛嘉琳，瞿涤
基金项目：教育部博士点基金新教师类（20100071120049） 作者简介：吴旸（1981-），男，讲师，研究方向：病原生物学 通信联系人：瞿涤（1951-），女，教授，研究方向：医学微生物学.

（复旦大学上海医学院医学分子病毒学教育部/卫生部重点实验室，上海 200032）
摘要：目的 检测色氨酰-tRNA合成酶抑制剂W-23对葡萄球菌的抗菌活性。方法 采用肉汤稀释方法检测W-23对葡萄球菌、大肠杆菌的最低抑菌浓度MIC，并检测其与氨苄青霉素或氯霉素联用对表皮葡萄球菌的联合抗菌效果。结果 化合物W-23对表皮葡萄球菌和金黄色葡萄球菌的MIC为2-4微克/毫升，抗菌类型属于抑菌剂，与氨苄青霉素联用对耐甲氧西林表皮葡萄球菌具有协同抗菌作用。结论 W-23对葡萄球菌具有较强的抗菌活性，具有潜在应用价值。
关键词：病原生物学；葡萄球菌；色氨酰-tRNA合成酶；抑制剂
中图分类号：R3

Antibacterial activity of a new inhibitor of Staphylococcus epidermidis tryptophanyl-tRNA synthetase
WU Yang, MAO Jialin, QU Di
(Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical School of Fudan University, Shanghai 200032)
Abstract: Objective: Investigate the antibacterial activity of W23, a new inhibitor of Staphylococcus epidermidis tryptophanyl-tRNA synthetase. Methods: Determine the Minimal Inhibitory Concentration of W-23 to Staphylococcus epidermidis, Staphylococcus aureus and Escherichia coli; detect the effect of W-23 in combination with Ampicillin or Chloramphenicol on S. epidermidis growth. Results: The MIC of W-23 to Staphylococcus species is about 2-4 μg/ml.  W-23 is a bacteristatic agent. Combination of W-23 and ampicillin has synergistic effect on growth of methicillin-resistent Staphylococcus epidermidis. Conclusion: W-23 has potent antibacterial activity against Staphylococcus and has a potential value in new drug development.
Key words: Medical Microbiology; Staphylococcus; tryptophanyl-tRNA synthetase；inhibitor