大气细颗粒PM2.5诱发支气管的高反应性
王荣1，肖雪1，曹蕾1，沈振兴2，曹永孝1
作者简介：王荣(1981-),女,博士生,主要研究方向:血管药理学 通信联系人：曹永孝（1957-），男，教授，血管药理学.

（1. 西安交通大学药理学系，西安，710061；2. 西安交通大学环境科学与工程系，西安，710049）
摘要：目的: 探讨大气细颗粒PM2.5的二甲基亚砜溶解物对大鼠支气管收缩功能的影响。方法：分离大鼠二级支气管，制成约1 mm长的环段，进行离体器官培养，培养后的支气管环采用小血管张力记录的方法，在组织浴槽中通过累加浓度给药法分别加入M胆碱受体激动剂卡巴胆碱，建立相应的收缩的时效和量效曲线。结果：卡巴胆碱能浓度依赖性地收缩新鲜的大鼠支气管。与新鲜组相比，支气管在单纯培养12h和24h，卡巴胆碱诱导的支气管收缩反应的Emax和pEC50均无显著性差异。而培养48h后，卡巴胆碱收缩支气管的量效曲线明显左移，收缩反应的Emax和pEC50均P＜0.01。与DMSO溶剂对照组相比，PM2.5培养12、24、48h均能增强卡巴胆碱引起的收缩反应，且培养24 h达到最大收缩反应。与单纯培养相比，支气管环在0.3、1.0或3.0 μg/ml PM2.5 中培养24h，均能增强卡巴胆碱引起的收15 缩反应，0.3，1.0 和 3.0 μg/ml PM2.5 组收缩反应的Emax 分别为 149.4±9.9%， 195.4±10.0%，和143.95±7.7%。结论：PM2.5增强了M受体介导的支气管的收缩作用，从而诱发大鼠支气管的高反应性。
关键词：PM2.5；大鼠；支气管；高反应性
中图分类号：R122.7 20
DMSO-Soluble Particular Matter (PM2.5 ) induces airway contractile hyperresponsiveness
WANG Rong1, XIAO Xue1, CAO Lei1, SHEN Zhenxing2, CAO Yongxiao1
(1. Xi’an Jiaotong University College of Medicine,Xi’an, 710061;2. Department of Environmental Science and Engineering,Xi’an Jiaotong University,Xi’an, 710049)
Abstract: Objective: To explore the effects of DMSO-Soluble Particular Matter (PM2.5 ) on the contractile function in isolated rat bronchial segments. Methods: The rat bronchi were dissected and cut into about 1 mm long segments. The bronchi were cultured in the different dose of PM2.5 30 and different hours. The contractile responses to carbachol (M receptor agonist) were recorded using a sensitive myograph system. Results: Carbachol induced an dose-dependent contraction in rat bronchial segments. Compared to fresh, organ culture with DMSO for 12h or 24h, carbachol induced contraction response did not significant difference. organ culture with DMSO for 48h induced significant contraction and Emax和pEC50 P＜0.01. Compared to DMSO, organ 35 culture with PM2.5 for 12, 24 or 48h, contraction response were enhanced by carbachol in rat bronchial segments. Compared to DMSO, bronchial segments cultured with 0.3, 1.0 or 3.0 μg/ml PM2.5 for 24h，contraction response were enhanced by carbachol. Emax were 149.4±9.9%, 195.4±10.0% or 143.95±7.7%, respectively。Conclusion: DMSO-Soluble Particular Matter (PM2.5 ) increased the M receptor-mediated contractile responses in rat bronchi and induced 40 airway hyperresponsiveness.
Key words: PM2.5; Rat; Bronchial segments; Hyperresponsiveness