前列腺素PGE2 对肝癌细胞CD29 表达调控及相关机制的研究
王洁，白小明，冷静
基金项目：高等学校博士学科点专项科研基金资助（20113234120009）
作者简介：王洁(1986),女,研究生,主要研究方向:PGE2 对肝癌细胞侵袭能力的影响
通信联系人：冷静(1954),男,教授,主要研究方向:PGE2 对肿瘤细胞生长与扩散的影响及其机制.

 （南京医科大学病理学系,南京 210029）
摘要：目的：阐明前列腺素E2(prostaglandin E2, PGE2)通过EP1 受体上调肝癌细胞Huh-7 中CD29 的表达及其相关的信号转导通路。方法：用PGE2、EP1 受体激动剂（17-phenyltrinor Prostaglandin E2，17-PT-PGE2）、EP1 受体抑制剂SC19220、NF-κB 抑制剂PDTC 处理Huh-7 细胞，通过Western blot、免疫荧光实验等方法检测CD29 蛋白表达水平和NF-κB 的活性。结果：5μmol/L 的PGE2 处理Huh-7 细胞24h 后，CD29 的表达水平与对照组相比上升了129.48% (P<0.01)，5μmol/L EP1 受体激动剂17-PT-PGE2 处理后使细胞CD29 的蛋白表达水平升高了216.34%（P<0.01）。10μmol/L 的EP1 受体抑制剂SC19220 处理后CD29 表达水平与PGE2 组相比下降了79.19%(P<0.05)。免疫荧光实验显示17-PT-PGE2 处理Huh-7 细胞120min 后，NF-κB 核表达水平明显增加；Western blot 实验显示Phospho-NF-κB-P65 水平明显增高。NF-κB 抑制剂PDTC 处理Huh-7 细胞后CD29 蛋白表达水平与EP1 受体激动剂组相比降低了94.95%(P<0.01)。结论：PGE2 可通过EP1 受体上调Huh-7 细胞中CD29 的表达，此调节作用可能与NF-κB 信号转导通路有关。
关键词：病理学；前列腺素E2；EP1 受体；CD29；NF-κB
中图分类号：R735.7

The role of PGE2 on CD29 expression and its related mechanism in hepatocellular carcinoma
WANG Jie, BAI Xiaoming, LENG Jing
(Department of Pathology, Nanjing Medical University, Nanjing 210029)
Abstract: Objective: To investigate the effect of prostaglandin E2 (PGE2) on the expression of CD29 and its related signaling pathway by EP1 receptor in Huh-7 cells. Methods: Huh-7 cells were treated with PGE2, EP1 receptor agonist（17-phenyltrinor Prostaglandin E2）, EP1 receptor antagonist SC19220,NF-κB inhibitor PDTC,Western blot and immunofluorescence test
were employed to detect the expression of CD29 and activation of NF-κB in Huh-7 cells.Results:The level of CD29 was increased by 129.48%(P<0.01) after being treated with 5μmol/L PGE2 for 24h, The level of CD29 was increased by 216.34%（P<0.01）after being treated with EP1 receptor agonist (5μmol/L ) for 24h. the expression level of CD29 was decreased by 79.19%(P<0.05) after being treated with EP1 receptor antagonist SC19220 (10μmol/L). The expression lever of cell nucleus was obviously increased for 120min by immunofluorescence; The level of 35 Phospho-NF-κB-P65 was increased through Western blot. After the treatment of NF-κB inhibitor PDTC , the level of CD29 was decreased by 94.95% (P<0.01). Conclusion: PGE2 might up-regulate the expression level of CD29 through EP1 receptor in Huh-7 cells, which could be partly related to the NF-κB signaling pathway.
Key words: Pathology; PGE2; EP1 receptor; CD29; NF-κB